DETERMINATION OF THE ED50 AND ANTINOCICEPTIVE ACTIVITY OF MAHALEB CHERRY (PRUNUS MAHALEB L.) IN MICE

Abstract

Herbs are the main part of traditional medical and dental practice. P. mahaleb L., a source of compounds useful for pharmaceutical industries. Aim of research to evaluate the antinociceptive effect of P. mahaleb L. Forty albino mice were used. P. mahaleb L. powder was given for animal orally at dose of (1, 5, 3, 12, 24) mg/kg. The antinociceptive effect was estimated by Hot-plate test. Median effective dose (ED50) of P. mahaleb L., determined by using up & down method. Difference between groups were done by one way analysis of variance and the least significant difference test (LSD). P. mahaleb L. at (3, 12, 24) mg/kg produce analgesic effects and increased in threshold of pain between pre and post treatment following 10 minute in comparison with 1.5 mg/kg at (6±0.0) second and control at ( 7.0 ± 0.0) second group respectively. The analgesic effect of P. mahaleb L. after 20 minutes of administration significantly difference between pre and post treatment in dose (12, 24) mg/kg (10 ±1.15) (12 ±1.1) sec respectively in comparison with other lower doses (1.5, 3) mg/kg and control. P. mahaleb L. not produce a dose dependent analgesic effect in mice. The pain reaction time was increased after 2 min and the maximum analgesic effect after 10 min of oral P. mahaleb L. administration in dose (3, 12, 24) mg/kg and duration of analgesia last from 2 min to 20 min. P. mahaleb L. possess analgesic activity, it can be useful as a good natural source of compound in pharmaceutical industry. Keywords: Prunus mahaleb L., Analgesic, Albino Mice, ED50.